Toxicological Analysis of FeNPs through Behavioral Examination by Using Head Dip Test

Abstract

Iron nanoparticles are applicable in the delivery of drugs, cancer treatment, and magnetic resonance imaging, all while demonstrating a non-toxic effect. It is crucial to ensure the potential toxicity by comprehensive assessment of iron nanoparticles in human behavioral health through rabbit models. The aim of the study was to investigate validity of neophilia on the hole-board task over repeated sessions about 1-15 trials. It was observed that potential toxicity varies on the concentration level of iron nanoparticles i-e; 20mg/kg, 40mg/kg, 60mg/kg, 80mg/kg, 100mg/kg. It is also observed that increase in concentration of iron nanoparticles results in decrease in neophilia and hence proves more toxicity. The rabbits were categorized into six randomly divided groups, each group contains two male and 2 female rabbits. All the rabbits were placed for 2 weeks in standard environmental condition and after two weeks all the rabbits were randomly divided into five experimental groups named as E1, E2, E3, E4, and E5 with an additional control group as E0. E denoted as experimental group. In this study dosage of iron nanoparticles were given to rabbits through intravenous and oral administration in total duration of 5 weeks. Examination of behavioral patterns was studied throughout the trial period at equal intervals of time caused by potential toxicity of FeNPs on the physiology of the rabbits. To validate the findings calculated data was subjected to statistical analysis. It was concluded that number of head dips were decreasing and fatal in the activity of animal were observed due to physiological effect of iron nanoparticles on the brain or motor skills of the rabbit. Overall, the study proves the potential toxicity of iron nanoparticles in the rabbit model.