Assessment of Melia Azedarach Leaf Extract on Growth, Immune Responses, and Hematological Profile of Cyprinus Carpio
DOI:
https://doi.org/10.63163/jpehss.v4i1.1319Keywords:
Common Carp, Melia azedarach, Immunological parameters, Hematology, ImmunityAbstract
The objective of this study was to examine the effect of Melia azedarach, commonly known as chinaberry, on the growth, hematological characteristics, and serum immunological parameters of Common carp (Cyprinus carpio). This experimental study involved four groups: a control group fed with a basal diet having no Melia azedarach leaf extract addition, and three experimental groups, T1, T2, and T3, fed with 1%, 2% and 3% of Melia azedarach leaf extract, respectively. Each aquarium contained ten fish. The results obtained at the end of the feeding period indicated that the Common carp fed with diets comprising 1%, 2% and 3% Melia azedarach leaf extract had higher weight gain, SGR percentage, and feed conversion ratio compared to the control group. Hematological analyses revealed that Red blood cell (RBC) count (Control: 2.50×106/µL; T3: 4.15×106/µL) and hemoglobin (Hb) levels (Control: 6.80×µL; T3: 11.20µL) were high. Similarly, immunological parameter were also high in all treatment group i.e. Neutrophil (Control: 80%; T3: 85.5), Lymphocytes (Control: 6%; T2 and T3:8), Monocytes (Control: 3%; T2: 3.9) and Eosinophil (Control: 3%; T3: 2,4) were significantly higher in the treatments groups compare to the control group, suggesting a positive growth and health effect of the plant extract on grass carp. The study concludes that Melia azedarach leaf extract has a positive effect on the growth, hematology, and immunological parameters of common carp, therefore, safe in the current proportion as investigated in this experiment. Also, a cost-effective natural growth promoter and immunity booster being easily available in the local markets of Pakistan, Melia azedarach may as well be included in the diets of common carp in fish hatcheries across the country.
References
Al-Rubae, A. Y. (2009). "The potential uses of Melia azedarach L. as pesticidal and medicinal plant, review." American-Eurasian Journal of Sustainable Agriculture 3(2): 185-194.
Anderson, J. L., et al. (2017). Aquaculture: Its role in the future of food. World agricultural resources and food security: International food security, Emerald Publishing Limited: 159-173.
Asadujjaman, M., et al. (2013). "Assessment of bioactivities of ethanolic extract of Melia azedarach (Meliaceae) leaves." Journal of Coastal Life Medicine 1(2): 118-122.
Awad, E. and A. Awaad (2017). "Role of medicinal plants on growth performance and immune status in fish." Fish & shellfish immunology 67: 40-54.
Béné, C., et al. (2015). "Feeding 9 billion by 2050–Putting fish back on the menu." Food security 7(2): 261-274.
Chopin, T., et al. (2001). "Integrating seaweeds into marine aquaculture systems: a key toward sustainability." Journal of phycology 37(6): 975-986.
Citarasu, T. (2010). "Herbal biomedicines: a new opportunity for aquaculture industry." Aquaculture International 18(3): 403-414.
Galindo-Villegas, J. and H. Hosokawa (2004). "Immunostimulants: towards temporary prevention of diseases in marine fish." Avances en nutricion acuicola.
Greathead, H. (2003). "Plants and plant extracts for improving animal productivity." Proceedings of the nutrition Society 62(2): 279-290.
Guo, J., et al. (2021). "Lead impaired immune function and tissue integrity in yellow catfish (Peltobargus fulvidraco) by mediating oxidative stress, inflammatory response and apoptosis." Ecotoxicology and Environmental Safety 226: 112857.
Immanuel, G., et al. (2009). "Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus." Journal of fish biology 74(7): 1462-1475.
JAVED, Y., et al. (2012). "Comparative efficacy of different schedules of administration of medicinal plants mixed infusion on hematology of broiler chicks." Sarhad Journal of Agriculture 28(2).
Matiullah, F. R., et al. (2016). "Use of black pepper seed as growth enhancer in Labeo rohita." International journal of biosciences 8(3): 1-9.
Midtlyng, P. (1997). "Protection versus side-effects." Developments in biological standardization 90: 371-379.
Naylor, R. L., et al. (2000). "Effect of aquaculture on world fish supplies." Nature 405(6790): 1017-1024.
Parkos, J. and D. Wahl (2014). "Effects of common carp (Cyprinus carpio), an exotic fish, on aquatic ecosystems. Ilinois Natural History Survey report of January/February 2000. University of Illinois Board of Trustees." Center for Aquatic Ecology.
Pratheepa, V. and N. Sukumaran (2014). "Effect of Euphorbia hirta plant leaf extract on immunostimulant response of Aeromonas hydrophila infected Cyprinus carpio." PeerJ 2: e671.
Ramya, S., et al. (2009). "In vitro antibacterial prospective of crude leaf extracts of Melia azedarach Linn. against selected bacterial strains." Ethnobotanical Leaflets 2009(1): 32.
Reid, G. K., et al. (2019). "Climate change and aquaculture: considering biological response and resources." Aquaculture Environment Interactions 11: 569-602.
Reverter, M., et al. (2014). "Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives." Aquaculture 433: 50-61.
Sen, A. and A. Batra (2012). "Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L." Int J Curr Pharm Res 4(2): 67-73.
Sharma, D. and Y. Paul (2013). "Preliminary and pharmacological profile of Melia azedarach L.: An overview." Journal of Applied Pharmaceutical Science 3(12): 133-138.
Yaqoob, S. (2021). "A Review of Structure, Origin, Purpose & Impact of Common Carp (Cyprinuscarpio) in India." Annals of the Romanian Society for Cell Biology 25(6): 34-47.
