Dietary Selenium Supplementation Alleviated High Concentrate Diet-Induced Lipopolysaccharide Concentration by Improving Antioxidative Status in Goats

Abstract

Antioxidant mechanisms and the prevention of cellular damage depend heavily on selenium (Se). Goats fed high concentrate (HC) diets experience oxidative stress as a result of aberrant rumen fermentation, which raises the concentration of lipopolysaccharides (LPS). This study evaluated the effect of dietary Se on LPS concentration and antioxidant activity in blood serum of goats fed HC diet. Thirty cross-bred goats (12-16 weeks, 11-13 kg) were randomly kept in three groups: LC (low concentrate diet), HC (high concentrate diet), and HC-SY (HC + Se-yeast @ 0.5 mg/kg diet) for 10 weeks. LPS content and oxidative stress biomarkers such as malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAC) were determined in blood serum. LPS concentration was decreased (P < 0.001) in HC-SY than HC group. Antioxidant status of oxidative stress biomarkers improved with a significant decrease (P < 0.001) of MDA level in HC-SY compared to HC. GSH-Px activity decreased in HC as compared LC, but it escalated (P < 0.001) in HC-SY compared to other groups. SOD activity reduced in group HC However, it significantly increased (P < 0.001) in Se supplemented group than in HC. CAT activity increased (P < 0.01) in group HC-SY than in HC but it was non-significant (P > 0.05) among LC and HC. TAC activity increased (P < 0.05) in HC-SY than HC. In conclusion, HC diet supplemented with dietary Se showed ameliorative effects in blood serum by enhanced antioxidant activity and alleviated LPS content in goats compared to HC diet.