Isolation, Identification, and Characterization of Escherichia coli from Urinary Tract Infection Patients Using Conventional and Advanced Diagnostic Technique

Abstract

The main aim of this study was the isolation, identification and detection of Escherichia coli (E. coli) among Urinary Tract Infection (UTI) Patients. Data was collected from UTI patient visiting Out Patients Department (OPD), Hayatabad Medical Complex (HMC). A total of 60 patients, both male and female, were included in the study. All the samples were cultured on microbiological medium. For isolation, CLED agar media was utilized while for purification the study used MacConkey agar media. Identification of E. coli was done on the basis of morphological characteristics, gram staining and biochemical tests i.e. Indole, Urease, Catalase, Oxidase, Citrate, and Triple Sugar Iron (TSI), Methyl red, Eosin Methylene Blue Agar media tests were performed. ESBL detection was done according to Double Disc Synergy test. The results of the study indicated that out of 60 samples, 42 (70 percent) patients were E. coli positive. E. coli was positive and higher among married patients as compare to unmarried patients. ESBL was positive among 33 patients while negative among 09 patients. The E. coli was resistant to ATM among 14.29 percent while it was sensitive to ATM for the remaining 85.21 percent samples. The E. coli was resistant to AMC among 21.43 percent and sensitive to AMC for 78.57 percent sample. The lowest resistant was shown against ATM with 12.12 percent while AMC are 100 percent sensitive against positive E. coli. CFM are lowest second number resistant showing 27.27 percent resistant followed by NOR and CIP which are 33.33 percent and 39.39 percent resistant, respectively. NOR, CIP, CAZ, CTX and AMC are 100 percent resistant against2 negative E. coli, while CFM are 88.89 percentresistant to negative E. coli. ATM is less resistant antibiotic having only 22.22 percent resistivity.